Received: OctoAccepted: FebruPublished: March 18, 2014Ĭopyright: © 2014 Martin et al. PLoS ONE 9(3):Įditor: Carlos Eduardo Ambrosio, University of São Paulo, Brazil
(2014) Mesenchymal Stem Cells Do Not Prevent Antibody Responses against Human α-L-Iduronidase when Used to Treat Mucopolysaccharidosis Type I. Therefore, the humoral immunosuppressant property of MSC is questionable and indicates the danger of using MSC as a source for the production of exogenous proteins to treat monogenic diseases.Ĭitation: Martin PKM, Stilhano RS, Samoto VY, Takiya CM, Peres GB, da Silva Michelacci YMC, et al. On the contrary, these cells worked as an adjuvant that favored IDUA immunization. In conclusion, the antibody response against IDUA could not be avoided by MSC. The spreading of the transplanted MSC into the peritoneum of other organs was confirmed after injection of 111In-labeled MSC. MSC-transplanted mice had high levels of TNF-alpha and infiltrates in the renal glomeruli. The antibody titers were high and comparable to mice that were immunized by electroporation. Anti-IDUA antibody response was also detected in C57Bl/6 mice treated with MSC-WT-IDUA. After the third cell transplantation, a high titer of anti-IDUA antibody was detected in all of the treated mice. Only after the second cell transplantation, more than one unit of IDUA activity was detected in the blood of 3 mice for 2 days. The total IDUA activities from MSC-KO-IDUA before cell transplantation were 9.6, 120 and 179 U for the first, second and third injections, respectively. For cell transplantation, 4×10 6 MSC-KO-IDUA cells (MSC from KO mice modified with IDUA) were injected into the peritoneum of KO-mice three times over intervals of more than one month. Sleeping Beauty transposon vectors were used to modify MSC because these are basically less-immunogenic plasmids. Due to the immunosuppressant properties of MSC, we hypothesized that MSC modified with the IDUA gene would be able to produce IDUA for a long period of time. Enzyme replacement therapy is recognized as the best therapeutic option for MPSI however, high titers of anti-IDUA antibody have frequently been observed. Mucopolysaccharidosis type I (MPSI) is an autosomal recessive disease that leads to systemic lysosomal storage, which is caused by the absence of α-L-iduronidase (IDUA).
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